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@# Objective: : To investigate the expressions of chemokine-like factor superfamily 6 (CMTM6) and programmed cell death ligand 1 (PD-L1) in glioma tissues and their correlation with clinicopathological features of patients. Methods： ：From January 2012 to December 2015, 86 brain glioma tissues and 30 brain tissues (Control group) from patients operated with decompressive of craniotomy were collected from the FifthAffiliated Hospital of Zhengzhou University. The distribution and expressions of CMTM6 and PD-L1 protein in brain glioma tissues were detected by immunohistochemistry and WB methods. The differential expression of CMTM6 and PDL1 between glioma tissues and normal brain tissues was analyzed by t test of two independent samples. Single variant χ2 test was used to analyze the relationship between the expression of CMTM6, PD-L1 and the clinicopathological features of patients. Results: The expression of CMTM6 in glioma tissues was significantly higher than that in control tissues (P<0.01). The expression levels of CMTM6 and PD-L1 in high pathological grade (WHO III-IV) glioma tissues were significantly higher than those in low pathological grade (WHO I-II) glioma tissues (all P<0.01). The expression of CMTM6 was correlated with pathological grade, dizziness history, epilepsy seizure and PD-L1 expression (all P<0.05), while the expression of PD-L1 was correlated with pathological grade, epilepsy seizure and CMTM6 expression (all P<0.05). Conclusion: There is a correlation between the expression of CMTM6 and PD-L1 in glioma tissues, both of which are highly expressed and are expected to be used to study glioma signaling pathways.
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Objective To evaluate the expression of trefoil peptides (TFF1 ,TFF3) in colonic polyps and its relationship with clinicopathological parameters.Methods 120 cases of colon polyps were selected as the colon polyps group ,including 40 cases of hyperplastic polyps and 80 cases of adenomatous polyps ,and 30 cases of co-lon cancer (colon cancer group) and 20 cases of normal colonic mucosa (normal colon mucosa group) were se-lected as controls.The expression of TFF1 and TFF3 in various tissues were detected by q-RT-PCR ,Western Blot and immunohistochemistry ,and their relationship with clinicopathological parameters was analyzed.Re-sults The positive rates of TFF1 in normal colon mucosa ,hyperplastic polyp tissue ,adenomatous polyp and colon cancer were 0 ,0 ,53.8% and 80.0%,respectively and the differences were statistically significant (χ2=66.614 ,P＜0.05).The positive rates of TFF3 in normal colon mucosa ,hyperplastic polyp tissue ,adenomatous polyp and colon cancer were 90.0%,77.5%,55.0% and 30.0%,respectively.and the differences were statisti-cally significant (χ2=24.688 ,P＜0.05).The differences of TFF1 mRNA ,TFF3 mRNA ,TFF1 protein ,TFF3 protein expressions in the four groups were statistically significant.TFF1 protein expression in moderate and severe dysplasia polyp tissues increased significantly compared with those in mild dysplasia polyp tissues (t=2.760 ,P=0.009) ,while the expression of TFF3 significantly decreased (t=2.556 ,P=0.015) ;the expression of TFF1 protein in villous adenomatous polyp tissues increased significantly compared with that in tubular ad-enoma tissues (t=2.549 ,P=0.013) ,while the expression of TFF3 protein decreased significantly (t=2.108 , P=0.038).Conclusion The expression of TFF1 and TFF3 is closely related to the process of malignant co-lonic cancinogenesis ,and can be used as a biomarker for the differential diagnosis of colon benign diseases and early diagnosis of colon cancer.
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Objective To study the expression of HDAC2 and the correlations with pathological parameters in human tongue squamous cell carcinoma, and to analyze the correlation between them and patients' clinical indicators and survival status.Methods A total of 96 cases of humantongue squamous cell carcinoma (SCC), 24 dysplasia (ED), 13 normal mucosa (NOM) were involved in this study. The expression of HDAC2 was evaluated by ICH. Kaplan-Meier was used to research cumulative surviving of SCC patients.Result Among 96 cases of SCC samples, 86 cases showed positive expression (89.6％), The differences between the overexpression of HDAC2 and the pathological grading, lymph node metastasis, the patient's age and gender were insignificant (all P＞0.05). However, the differences of TNM presented statistically significant (χ2 = 18.600, P＜0.001). 14 out of 24 ED (58.3％) were positive expression of HDAC2, there were 7 cases (53.8％) of positive expression samples in NOM. The expression of HDAC2 in SCCs was significantly higher than that in ED and NOM (χ2= 118.407, P＜0.00). There was no significant difference of cumulative surviving between the positive and negative SCC patients (P = 0.184). However cumulative surviving of patients with lymph node metastasis was significantly shorter than those with no metastasis (P＜0.000).Conclusions The expression of HDAC2 in SCC was significantly higher than that in ED and NOM, the TNM stage of patients presented the significant difference of expression of HDAC2, patients with lymph node metastasis were significantly shorter than those with no metastasis, and demonstration can be used as a reliable reference molecule for the evaluating prognosis of SCC.
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Objective To explore the expression of human sodium coupled neutral amino acid transporter 1 (SNAT1) in human glioma tissues and its relationship with clinical pathological parameters and prognosis. Methods Immu?nohistochemical and western blotting were used to detect SNAT1 expression in glioma tissue and tumor peripheral tissue from 89 cases of glioma patients including 55 cases of low grade gliomas (WHO I-II), and 34 cases of high grade gliomas (WHO grade III-IV).χ2 test and was used to analyze the relationship between expression and clinical pathological param?eters of SNAT1. Kaplan-Meier method was used to analyze the effect of different expression of SNAT1 on the prognosis of patients and to establish the Cox regression model. Results The expression of SNAT1 was significantly higher in gliomas than in tumor peripheral tissue (t=-9.803, P=0.001). The expression of SNAT1 was significantly higher in high pathologi?cal grade tissues than in low grade of glioma tissues (t=-6.682, P=0.003). SNAT1 expression was associated with tumor di?ameter and pathological grade (χ2=4.963, 8.527, P<0.05);Cox regression model showed that the tumor pathological grade and different SNAT1 protein expression were independent risk factors for the prognosis of patients with glioma. Conclu?sions The expression of SNAT1 protein is closely associated with the pathological grade of gliomas and the prognosis of the patients, which may be a new target to judge the biological characteristics and to evaluate the prognosis of gliomas.
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Objective To investigate the expression level and clinical significance of p53 ,C-myc and CerbB-2 in papillary thy-roid carcinoma patients .Methods The expression level of p53 ,C-myc and CerbB-2 were detected in 45 cases of papillary thyroid carcinoma tissue specimens and 45 cases of nodular goiter tissue specimens by using immunohistochemical staining .Their correla-tions with clinic pathological features in papillary thyroid carcinoma were analyzed further .Results The positive expression rates of p53 ,C-myc and CerbB-2 in the papillary thyroid carcinoma tissue specimens were significantly higher than that of the nodular goiter tissue specimens(all P< 0 .01) .The expression of C-myc and CerbB-2 significantly correlated with lymph node metastasis(P< 0 . 05) .The expression of CerbB-2 significantly correlated with tumor size ,local infiltration ,TNM stage ,differentiated degree and lymph node metastasis (all P< 0 .05) .p53 positive expression ,C-myc positive expression and CerbB-2 positive expression were the independent factors of cervical lymph node metastasis .Conclusion p53 ,C-myc and CerbB-2 were highly expressed in papillary thy-roid carcinoma and correlated with cervical lymph nody metastasis .The detection of p53 ,C-myc and CerbB-2 might be helpful for diagnosis of papillary thyroid carcinomas .
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We conducted a retrospective study of 41 cases of clinically localized renal cell carcinoma (RCC) treated with radical nephrectomy by our department between January 1987 and December 1993. The prognostic pathological parameters considered were tumor extension (pT stage), nuclear grading, histological grading, cell type, histologic growth pattern and tumor size. In the univariate analysis by log-rank test, three parameters showed prognostic significance, including pT stage, nuclear grading and tumor size. However, in the multivariate analysis using Cox's regressional hazard model, only two parameters including pT stage (p0.05). We think that nuclear grading cannot predict the outcome of patients at the same surgical stage. These results suggest that pathological stage of RCC may be the most important prognostic factor and the nuclear grade of tumor may provide additional important prognostic information.